Insertion junction: LMJ.RY0402.143214_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.143214
Chromosome:chromosome 8
Location:834696
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g360950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCACTCCCGCTCTCGGTGCGCACGCGGT
Internal bar code:CGGTTACGCCCCCCTTGGCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:82
LEAP-Seq percent confirming:99.8703
LEAP-Seq n confirming:770
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTGCATGTCACTGCACCT
Suggested primer 2:CAGCAAGTCCAAGAAGGAGG