| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143215 |
| Chromosome: | chromosome 3 |
| Location: | 3945551 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171650 | HEL16 | (1 of 2) PTHR18934:SF145 - ATP-DEPENDENT RNA HELICASE DHX57-RELATED; DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGCGCGTTCATGAAGCTGGTGTCGTAG |
| Internal bar code: | TGTCGGCACATGGAGTCGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 95.0629 |
| LEAP-Seq n confirming: | 5064 |
| LEAP-Seq n nonconfirming: | 263 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACGG |
| Suggested primer 2: | GGCATGAGTCAGGAAATGGT |