| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143221 |
| Chromosome: | chromosome 16 |
| Location: | 6641624 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671700 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCTTGGAGCCCACACGCGGCATGGGCT |
| Internal bar code: | GGGCGCCGCCGGGTGCCATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 89.5789 |
| LEAP-Seq n confirming: | 851 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTGCACATATCAGGAGA |
| Suggested primer 2: | CTCAACCATCCTGCTGTTCA |