| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143225 |
| Chromosome: | chromosome 12 |
| Location: | 1439333 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487850 | CGL49,ARL11 | (1 of 1) K07977 - Arf/Sar family, other (ARF); ARF/SAR superfamily small monomeric GTP binding protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCCCCCTGCACCACGGCCAGTAATGAC |
| Internal bar code: | GATGATGGCGGAGAATAAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 99.1282 |
| LEAP-Seq n confirming: | 3070 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGGGCTTCGTATTTGTA |
| Suggested primer 2: | GCCCTCGGGCTTATAACTTC |