| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143232 |
| Chromosome: | chromosome 4 |
| Location: | 1270867 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215001 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGGGAGAGGCAGTAGAAGGGAGGGAGC |
| Internal bar code: | CCACCGCTTCGACTGATACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 454 |
| LEAP-Seq percent confirming: | 99.3912 |
| LEAP-Seq n confirming: | 5551 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGACCCATTTACCAACCA |
| Suggested primer 2: | TGCAGCTGGTATGGGTGTTA |