| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143233 |
| Chromosome: | chromosome 12 |
| Location: | 3902344 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515901 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGCTGCCGCGCCATCCACTGCACCTCC |
| Internal bar code: | GACGGGGGGCTGACATAGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 58.2895 |
| LEAP-Seq n confirming: | 443 |
| LEAP-Seq n nonconfirming: | 317 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGCATGGTAGAAGGTCC |
| Suggested primer 2: | CAGGTCTTCCCGTGATGTCT |