| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143252 |
| Chromosome: | chromosome 7 |
| Location: | 2193551 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327050 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTGCGCACGGACGCTCGTGCCCACCAG |
| Internal bar code: | TGCACGCTGGTCGGCGCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 85.6383 |
| LEAP-Seq n confirming: | 966 |
| LEAP-Seq n nonconfirming: | 162 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCAAGTGTGCAAGCTC |
| Suggested primer 2: | TTTCCAATCTTTCCCGTCAG |