| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143254 |
| Chromosome: | chromosome 7 |
| Location: | 116333 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313000 | (1 of 2) 2.7.7.48 - RNA-directed RNA polymerase / RNA nucleotidyltransferase (RNA-directed) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTAGCGTGAAGTGGGTTGGACGGACGAT |
| Internal bar code: | ATTCCACCAACGCGGTCGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 99.6789 |
| LEAP-Seq n confirming: | 3104 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCATGTGGTGAGGTTGGA |
| Suggested primer 2: | AGCTTGGACCCATACCTCCT |