| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143257 |
| Chromosome: | chromosome 2 |
| Location: | 1978864 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088200 | PDI1,cPDI,RB60 | (1 of 1) K09580 - protein disulfide-isomerase A1 (PDIA1, P4HB); Protein disulfide isomerase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCGCCCTAGGAACAAGAAGAGGTGGC |
| Internal bar code: | CTAGGTTCAAGTGTATCCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 98.1077 |
| LEAP-Seq n confirming: | 674 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTGGTTAGCGACCTGTG |
| Suggested primer 2: | GTCCTTGCTAGGAGACGCAC |