| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143263 |
| Chromosome: | chromosome 13 |
| Location: | 667571 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566250 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTAGCAGGTTTACAACAGGCGACCAGA |
| Internal bar code: | TAAGCCTTTAGGGCTTATATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 99.6651 |
| LEAP-Seq n confirming: | 2678 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCTACCGCAAGGTGTGT |
| Suggested primer 2: | AAGGCGTTCATGTGGGTTAG |