Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.143266 |
Chromosome: | chromosome_2 |
Location: | 5524834 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g108450 | FAP280 | Flagellar Associated Protein, transcriptional coactivator-like | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGAAGCACGCCGAAGCCAAGCACTTACGA |
Internal bar code: | GTGGCTCATAGGCATCATCGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 496 |
LEAP-Seq percent confirming: | 99.6789 |
LEAP-Seq n confirming: | 3415 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAAAGCATGGCTTACCCAC |
Suggested primer 2: | GGCTTCTCGTTGATAGCCTG |