| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143274 |
| Chromosome: | chromosome 8 |
| Location: | 3904991 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378200 | PPO1 | (1 of 1) 5.1.99.6 - NAD(P)H-hydrate epimerase / NAD(P)HX epimerase; Pyridoxamine 5'-phosphate oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATACGACGTGCGGGTGAGACAGGCCGCA |
| Internal bar code: | GGAGGCCCAGGTAGGTGATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGATCTTGGATTCAGTGT |
| Suggested primer 2: | ACAGCAAAACCCAAAAGCAC |