| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143293 |
| Chromosome: | chromosome 3 |
| Location: | 6219098 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192450 | CYA3 | Adenylate cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCTCAGGCCACGCCAGCTGCAGCCCC |
| Internal bar code: | CTCGGAACGAGCGGGCTCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 98.6532 |
| LEAP-Seq n confirming: | 293 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCCTTTTGGGCTGACAC |
| Suggested primer 2: | GCACGCGGTGTATAAAGGTT |