Insertion junction: LMJ.RY0402.143308_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.143308
Chromosome:chromosome 7
Location:4109477
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g342920 (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACAGCTGTGTCCGTACGGCACCGCCGCC
Internal bar code:CGCGACTGCGGGGCCCTCTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:210
LEAP-Seq percent confirming:99.829
LEAP-Seq n confirming:3502
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AAACTGAATCGAACCCAACG
Suggested primer 2:CACACACCTGTCCGTGTTTC