| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143318 |
| Chromosome: | chromosome 6 |
| Location: | 5064653 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280475 | (1 of 1) PTHR13018//PTHR13018:SF40//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // SUBFAMILY NOT NAMED // PHOSPHATE METABOLISM PROTEIN 7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTATTATTCAATAGAGTTCTTCTGAAGC |
| Internal bar code: | GAACGGACGCGGTTGAGCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 730 |
| LEAP-Seq percent confirming: | 99.9113 |
| LEAP-Seq n confirming: | 4506 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCGTAGGTGTGGTCTTC |
| Suggested primer 2: | GCTAGTCCCACAATCGGTGT |