| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143319 |
| Chromosome: | chromosome 3 |
| Location: | 814535 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146427 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGGGCTTCACTGCCGCCGCGGTGGAAC |
| Internal bar code: | AGGCCTAATTAGATCTCGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2512 |
| LEAP-Seq percent confirming: | 99.3315 |
| LEAP-Seq n confirming: | 2526 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGCCTCTGTTGACTAC |
| Suggested primer 2: | TGTGTGTGTGTTCCATGTGC |