| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143322 |
| Chromosome: | chromosome 10 |
| Location: | 2195814 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433800 | CSE21 | Predicted protein of CSE family | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGGTTGGATACATGTCGACGTTCGCGA |
| Internal bar code: | AGCCCGCCTGCGGCGTTCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 95.3734 |
| LEAP-Seq n confirming: | 4432 |
| LEAP-Seq n nonconfirming: | 215 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAACGGTGGCATTGATGGT |
| Suggested primer 2: | TATGCGAAACAGCGACTTTG |