| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143325 |
| Chromosome: | chromosome 9 |
| Location: | 3267612 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387949 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGTGCAGCACTGCCGTCACTCGCGCAG |
| Internal bar code: | GCCCTTAAAACACTAGTTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 97.7556 |
| LEAP-Seq n confirming: | 392 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACACAGGTTCAGCAGGAG |
| Suggested primer 2: | TCTGCGCAAATAGGAGGTCT |