| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143348 |
| Chromosome: | chromosome 1 |
| Location: | 7556580 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054500 | (1 of 1) 1.6.1.2 - NAD(P)(+) transhydrogenase (Re/Si-specific) / Transhydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGGCGCCGTGGCGCCAGCTCCGTCCAT |
| Internal bar code: | AAGCGCTAGCCACAGCGCAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 28 |
| LEAP-Seq percent confirming: | 99.7561 |
| LEAP-Seq n confirming: | 818 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCAACACCTTCATGTTC |
| Suggested primer 2: | AGCCAGACACAGCACAGTTG |