| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143361 |
| Chromosome: | chromosome 14 |
| Location: | 1329053 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616976 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCCTGTCCATTCTTGATGTGATGTGAT |
| Internal bar code: | GTGATGTAACGGTAAGGTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 770 |
| LEAP-Seq percent confirming: | 84.8047 |
| LEAP-Seq n confirming: | 2757 |
| LEAP-Seq n nonconfirming: | 494 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACCCACACTCAGTACCC |
| Suggested primer 2: | AAATGAAGTGGCAGGACAGG |