Insertion junction: LMJ.RY0402.143382_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.143382
Chromosome:chromosome 12
Location:3473055
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g496200 XPO2 Exportin-t; (1 of 1) K14288 - exportin-T (XPOT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGCTTACCTGCAGCACCGCCACCAGCG
Internal bar code:TTCTCGTTTTTCTTTCCCCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:169
LEAP-Seq percent confirming:83.2673
LEAP-Seq n confirming:1050
LEAP-Seq n nonconfirming:211
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGAGGTCAAGTTTTGGTG
Suggested primer 2:CACCCCTCTCCACACATTCT