| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143393 |
| Chromosome: | chromosome 8 |
| Location: | 3953351 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378650 | CGl131 | (1 of 18) IPR011051 - RmlC-like cupin domain; Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGGTGGACAGGCGGCGGGAACCGGAT |
| Internal bar code: | CGACGGTGAGAGAAGCGTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 94.4055 |
| LEAP-Seq n confirming: | 33378 |
| LEAP-Seq n nonconfirming: | 1978 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACCACAGCAATGACACC |
| Suggested primer 2: | GCTTCGTCAAGTTCGAGGTC |