Insertion junction: LMJ.RY0402.143467_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.143467
Chromosome:chromosome 14
Location:1074946
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g614850 FAP33,ANK17 Flagellar Associated Protein 33; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCCGCCATGCCCAGCAGGTGAGAGAGAG
Internal bar code:GAGTCACGGGCTTCAGGTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAATGAATGAATGTGTGCGG
Suggested primer 2:GAGCGCAACATCAAGTGGTA