| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143471 |
| Chromosome: | chromosome 16 |
| Location: | 2620466 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661800 | (1 of 1) PF04949 - Transcriptional activator (Transcrip_act) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCAGGCATGACTCGGTAGGGGTGGGGG |
| Internal bar code: | GACACTTACGGTGTTCAAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.7775 |
| LEAP-Seq n confirming: | 1794 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCTGCAATCAGGAACAT |
| Suggested primer 2: | CGTTTTGGGTGGAGTCAAGT |