| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143483 |
| Chromosome: | chromosome 3 |
| Location: | 6712310 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197700 | HLM8 | (1 of 1) PTHR13793//PTHR13793:SF100 - PHD FINGER PROTEINS // HISTONE-LYSINE N-METHYLTRANSFERASE ATX1-RELATED; Histone-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCCTGTGACCCCCTCTCGTGCGCCTCT |
| Internal bar code: | ACGCCCGATTTTGTCAACGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 98.5594 |
| LEAP-Seq n confirming: | 3626 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAGCAACCTTCTTCAGC |
| Suggested primer 2: | TAAGCAGCATGCTTTGTTGG |