| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143499 |
| Chromosome: | chromosome 14 |
| Location: | 3685159 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631650 | FAP406 | Flagellar Associated protein 406 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGCGTCCGTAGCTCAGTGGTGGCGTGT |
| Internal bar code: | TCTTGTCGCCGATGGAGAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 109 |
| LEAP-Seq percent confirming: | 99.4419 |
| LEAP-Seq n confirming: | 1069 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTCAACTTGACGAAGCG |
| Suggested primer 2: | CAGTGGTCTGTGCCTTGCTA |