Insertion junction: LMJ.RY0402.143503_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.143503
Chromosome:chromosome 16
Location:6913318
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g676757 PTB8 (1 of 9) K14640 - solute carrier family 20 (sodium-dependent phosphate transporter) (SLC20A, PIT); Sodium/phosphate symporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACTGCCACACACGTCTCAAACCCACGT
Internal bar code:CACGCTCTCTTCAGCAGACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:626
LEAP-Seq percent confirming:98.0985
LEAP-Seq n confirming:1135
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGCCCTTGATGTAACTGC
Suggested primer 2:GCCAAATGTACACTGCATGG