| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143513 |
| Chromosome: | chromosome 10 |
| Location: | 5805409 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGGTACGTCTCGAATCTGAACTCAGGC |
| Internal bar code: | CCGAAGAGTACCATAAGCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 58.8636 |
| LEAP-Seq n confirming: | 777 |
| LEAP-Seq n nonconfirming: | 543 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTTCCAGCCTCAGAAGA |
| Suggested primer 2: | AGCTCGTGTGCTGAGTGCTA |