Insertion junction: LMJ.RY0402.143514_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.143514
Chromosome:chromosome 3
Location:646555
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g145807 (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTAGATGCCACCCAGGCTCGCTTTTGT
Internal bar code:AGGTGTTGCCCCACAGGGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:99.9254
LEAP-Seq n confirming:5360
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTCCTGCTCCTCCTCCTC
Suggested primer 2:GTATTTATGCGGGGGAGGTT