| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143525 |
| Chromosome: | chromosome 16 |
| Location: | 1944876 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656300 | DPR1 | (1 of 2) 1.17.1.8 - 4-hydroxy-tetrahydrodipicolinate reductase / Dihydrodipicolinate reductase; dihydropicolinate (DAP) reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACGCCACCACCACCAAAAACAGTGTTT |
| Internal bar code: | TGGTATTGTCCATGGATTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1272 |
| LEAP-Seq percent confirming: | 99.7618 |
| LEAP-Seq n confirming: | 3770 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAAGCGTAAGCAGCCAGG |
| Suggested primer 2: | CGGTCCTGTAATTGGCTGTT |