| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143529 |
| Chromosome: | chromosome 12 |
| Location: | 2252279 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508000 | TIC40 | (1 of 5) IPR006636 - Heat shock chaperonin-binding; Putative chloroplast enveloppe protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTCAACCCGCTGCGCGCGGGTAGCCAC |
| Internal bar code: | CGGCAGGAAATAACAAGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 98.0673 |
| LEAP-Seq n confirming: | 103714 |
| LEAP-Seq n nonconfirming: | 2044 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTGTGTTGACTGTCGCT |
| Suggested primer 2: | GAGTGTGTATGCATGTCGGG |