Insertion junction: LMJ.RY0402.143536_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.143536
Chromosome:chromosome_11
Location:1702874
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre11.g467781 FAP151 ABC transporter-like and Flagellar Associated Protein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TCAGAGCACGGCCTCGATGCCGCATGCCGA
Internal bar code:GCGGTCGGCAAGTAAGGATTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:670
LEAP-Seq percent confirming:96.1105
LEAP-Seq n confirming:1705
LEAP-Seq n nonconfirming:69
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CTAACGCCAAGTTCGCTAGG
Suggested primer 2:AGTCTCGAATCCCCACACAC