| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143541 |
| Chromosome: | chromosome 12 |
| Location: | 513592 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493150 | LTO1,CPLD41,VKE1 | Vitamin K epoxide reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCCCACCACCATGTTGCTCGATTTGTC |
| Internal bar code: | GCTCCCACGTACCTTCAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 99.0969 |
| LEAP-Seq n confirming: | 6913 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGTGACAACCAAGCGACG |
| Suggested primer 2: | TCCTTCTCCACCTCATCCAG |