| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143552 |
| Chromosome: | chromosome 3 |
| Location: | 2435634 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159200 | UBC8 | (1 of 2) K10589 - ubiquitin-protein ligase E3 C (UBE3C); Putative ubiquitin-protein ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGCGAGTGCAAGGTCATGGATTTACA |
| Internal bar code: | CGTCCGCCCGACCGCGTTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 250 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGGTGGCAGTACAGAA |
| Suggested primer 2: | CTTTCGACCAACTAGCGAGG |