Insertion junction: LMJ.RY0402.143576_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.143576
Chromosome:chromosome 15
Location:1068682
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g638400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACCGAGGGGAAGCATTTCCTTGGATAG
Internal bar code:GTCGTTCGCTCAACCGTAGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:122
LEAP-Seq percent confirming:71.7391
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCGCACAACGTCTGATT
Suggested primer 2:GCGTGTTTGGTATGTGTTCG