| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143587 |
| Chromosome: | chromosome 6 |
| Location: | 1141786 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257050 | GBS2 | Granule bound starch synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTATAACCCCTCCCGCCGACATGTACATG |
| Internal bar code: | CAGGCGGGAGAACCACGTTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 97.9155 |
| LEAP-Seq n confirming: | 12448 |
| LEAP-Seq n nonconfirming: | 265 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAAATTCTTCGACCTTG |
| Suggested primer 2: | GACTTCCTGCTGGTGCCC |