| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143612 |
| Chromosome: | chromosome 12 |
| Location: | 6528955 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538850 | (1 of 1) PTHR13720//PTHR13720:SF9 - WD-40 REPEAT PROTEIN // ECHINODERM MICROTUBULE-ASSOCIATED PROTEIN-LIKE ELP-1; Similar to Echinoderm Microtubule Associated Protein Like 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTAGCGGCGTCAAGAGCGCTTGGGACAT |
| Internal bar code: | GGGGAAACATGGTGGGAGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 99.9349 |
| LEAP-Seq n confirming: | 4602 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACTTTCGAGTCTCGGTC |
| Suggested primer 2: | CAACTGGCCTGAGACATTGA |