| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143630 |
| Chromosome: | chromosome 17 |
| Location: | 3112523 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721350 | GST13 | Glutathione S-transferase; (1 of 6) K00799 - glutathione S-transferase (GST, gst) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCGTGATCAACGCCCGTCTACCGTGC |
| Internal bar code: | GGGAGGAGGCGAAGCTTCTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 99.8851 |
| LEAP-Seq n confirming: | 3478 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTACGTGAAGGGGCACAG |
| Suggested primer 2: | GGGACAGGGTAGGTCTCACA |