| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143631 |
| Chromosome: | chromosome 3 |
| Location: | 5435499 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185100 | (1 of 1) K15698 - RING finger protein 121 (RNF121) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCCCTCCATGCCCAGGCGCGCCGACAG |
| Internal bar code: | AGGCCTTACGGTTCGGATGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 35 |
| LEAP-Seq percent confirming: | 81.9749 |
| LEAP-Seq n confirming: | 4898 |
| LEAP-Seq n nonconfirming: | 1077 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCATACCACAAGCCAGCC |
| Suggested primer 2: | TGCGCTGTGATAAAGACCTG |