Insertion junction: LMJ.RY0402.143631_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.143631
Chromosome:chromosome 3
Location:5563684
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g186500 HAT2 Histone acetyltransferase 2; (1 of 1) K11303 - histone acetyltransferase 1 (HAT1, KAT1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCACGCCCCTGCATCCACCAGGTAACG
Internal bar code:ACTTAGGTTACTTACAATCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:527
LEAP-Seq percent confirming:68.1818
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTCATGCGTGGTCGTATGTT
Suggested primer 2:ATTTACTGGTAGGGGCTGGG