Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.143631 |
Chromosome: | chromosome 3 |
Location: | 5563684 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g186500 | HAT2 | Histone acetyltransferase 2; (1 of 1) K11303 - histone acetyltransferase 1 (HAT1, KAT1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCACGCCCCTGCATCCACCAGGTAACG |
Internal bar code: | ACTTAGGTTACTTACAATCATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 527 |
LEAP-Seq percent confirming: | 68.1818 |
LEAP-Seq n confirming: | 60 |
LEAP-Seq n nonconfirming: | 28 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCATGCGTGGTCGTATGTT |
Suggested primer 2: | ATTTACTGGTAGGGGCTGGG |