| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143640 |
| Chromosome: | chromosome 7 |
| Location: | 3718084 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337700 | PDC1 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTATGGTATAGCGGCGACATAGCGCAAA |
| Internal bar code: | TGTGCCAAAAAACGCCTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 98.4151 |
| LEAP-Seq n confirming: | 11612 |
| LEAP-Seq n nonconfirming: | 187 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTGGTGTAATGCAAGAG |
| Suggested primer 2: | TGGCAGCTGGTGAGTTACTG |