| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143641 |
| Chromosome: | chromosome 6 |
| Location: | 5528731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285001 | (1 of 1) PTHR34180//PTHR34180:SF1 - FAMILY NOT NAMED // MUTANT TAN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGGTGCGTGGTGGAGGACGAAGGCAG |
| Internal bar code: | GATTTCTCATTCTTTGAAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 68.5674 |
| LEAP-Seq n confirming: | 4810 |
| LEAP-Seq n nonconfirming: | 2205 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTCGCCCTGAGAGTTGC |
| Suggested primer 2: | CCTACCTGGGTCACGACAGT |