| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143649 |
| Chromosome: | chromosome 5 |
| Location: | 2226158 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234656 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGTGCTCTTGAGACGGCCGGACGCCCG |
| Internal bar code: | GCGGAGGACCCGAAAGGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 99.4872 |
| LEAP-Seq n confirming: | 1746 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATGGGGTTTTCTTGCAG |
| Suggested primer 2: | CGCGGACAAAACCTTGTTAT |