Insertion junction: LMJ.RY0402.143677_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.143677
Chromosome:chromosome 3
Location:8322349
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g199000 PHOT,PHT1,PHOT1 (1 of 1) PTHR24351//PTHR24351:SF76 - RIBOSOMAL PROTEIN S6 KINASE // PROTEIN TWIN LOV 1; Phototropin 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATACGTTCCGAACAGACCTCGCTGCTATT
Internal bar code:GGTACGGATAGTAATGAAGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:760
LEAP-Seq percent confirming:99.2853
LEAP-Seq n confirming:36814
LEAP-Seq n nonconfirming:265
LEAP-Seq n unique pos:116

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCGTTACACCGGACAAT
Suggested primer 2:AAGATGCTACTGATGCCGCT