| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143677 |
| Chromosome: | chromosome_3 |
| Location: | 8322349 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g199000 | PHT1,PHOT,PHOT1 | Phototropin | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATACGTTCCGAACAGACCTCGCTGCTATT |
| Internal bar code: | GGTACGGATAGTAATGAAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 99.2853 |
| LEAP-Seq n confirming: | 36814 |
| LEAP-Seq n nonconfirming: | 265 |
| LEAP-Seq n unique pos: | 116 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCGTTACACCGGACAAT |
| Suggested primer 2: | AAGATGCTACTGATGCCGCT |