| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143678 |
| Chromosome: | chromosome 6 |
| Location: | 1870588 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263100 | (1 of 2) PF10263 - SprT-like family (SprT-like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGTGTTCCCCTTGCCTCCCCCTCGTCA |
| Internal bar code: | TGGCTAAACTGGCGCACGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 903 |
| LEAP-Seq percent confirming: | 99.2966 |
| LEAP-Seq n confirming: | 5223 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTTCTGCCTGAACACTC |
| Suggested primer 2: | GGTGGGTGCGGTAGTTATTG |