Insertion junction: LMJ.RY0402.143681_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.143681
Chromosome:chromosome 8
Location:935389
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGATCAAAATCAACTGTGCACTCGCGATG
Internal bar code:GGCCGGTGAGCCCAACTGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1009
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGACCTCCACGTTGTCTT
Suggested primer 2:CTCTTCTTTCCCTGGCTGTG