| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143685 |
| Chromosome: | chromosome 3 |
| Location: | 2771341 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161950 | (1 of 26) IPR011990 - Tetratricopeptide-like helical domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGTACGGCACCCCCACCCCCCGCAGC |
| Internal bar code: | GGCACGAGACTAGATAGCCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1253 |
| LEAP-Seq percent confirming: | 98.9383 |
| LEAP-Seq n confirming: | 8946 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGAGTTTCGGCTGAGTTT |
| Suggested primer 2: | GTGTGGTGTCATGGTTGAGG |