| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143715 |
| Chromosome: | chromosome 16 |
| Location: | 5829909 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677900 | (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGGTCCGCCGCGTGCCACGTCCTCTTC |
| Internal bar code: | GCTTTCGGCGGGCCACGTCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 49 |
| LEAP-Seq percent confirming: | 98.0843 |
| LEAP-Seq n confirming: | 768 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGAAGAAGCCTCAGACG |
| Suggested primer 2: | TGTCCCCGCCTGTTATTTAG |