| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143739 |
| Chromosome: | chromosome 12 |
| Location: | 5423147 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530100 | CGL83 | (1 of 1) PTHR31446//PTHR31446:SF4 - FAMILY NOT NAMED // ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCGCCTCGCGTGGAAGCACCTGGTCTG |
| Internal bar code: | AGATTGGATCTGTGGGGTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 98.1506 |
| LEAP-Seq n confirming: | 1486 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAAGCTCAAAGAGGTGC |
| Suggested primer 2: | AGGTTGGCGAACATAAGGTG |