| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143748 |
| Chromosome: | chromosome 16 |
| Location: | 5311331 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682350 | (1 of 1) PTHR11685:SF154 - E3 UBIQUITIN-PROTEIN LIGASE ARI5-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTTGCCCGGACAAGCCTACTGTCCAGT |
| Internal bar code: | CGAAAACCCTTGGTCGGACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 98.744 |
| LEAP-Seq n confirming: | 5346 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGTCACGGGGCTGACAT |
| Suggested primer 2: | CATTTGTTACCACGCAGGTG |